Emerging BioTalk
  • Home
  • About Us
  • Contact
  • Search
  • Menu

Raman analytical monitoring of bioprocesses – a Process Analytical Technology (PAT) solution

September 20, 2021/in Bioprocessing, News /by Delphine Simiand

Cell culture processes are complex and highly variable in nature and yet only a handful of key parameters such as temperature, pH, and dissolved oxygen (DO) are typically controlled in real-time. While the measurement and control of these parameters is necessary for successful bioprocess monitoring, they do not provide a direct indication of the culture’s content itself, but only rough assumptions on the culture’s true state and limited process and cell growth understanding. Critical process parameters (CPP) such as glucose, lactate or ammonium and key performance indicators (KPI) such as total cell density (TCD) and viable cell density (VCD) provide a direct indication of the culture’s content and state but are generally measured off-line and therefore, not in real-time. Similarly, mammalian cell culture feeding strategies lack real-time measurement and rely on daily manual sampling which increases the risk of contamination and batch failures.

Our new Raman PAT Platform provides a path forward. Based on Raman technology, specifically designed for the bioprocessing industry, it enables to perform in-line and real-time measurement of CPPs and CQAs. It therefore helps improve processes, save time and provide flexibility to operators, reduce the risk of contamination and batch failures, and even implement a nutrient control loop strategy, a first step towards automation.

But what exactly is Raman technology, and why is it particularly well adapted to bioprocess monitoring?

The Raman effect

Raman technology in a non-linear process consisting of the exchange of energy between the optical wave and the molecular vibration of a substance.

For example, water (H2O) has three different molecular vibrations (bend, symmetric stretch, and asymmetric stretch) and one response of the molecular bond to excitation by a laser is the Raman signal. As the water molecule has three vibrations, there are three different Raman signals.

A Raman spectrum is a graph of Raman signal intensity as a function of the Raman shifts. For a water molecule, each of the three Raman signals correspond to a peak in the water Raman spectrum. A Raman spectrum can be likened to a molecular fingerprint.

Raman in bioprocessing

Raman technology is fully adapted for measurement in aqueous medium with no impact of water. It is non-destructive and can provide simultaneous, real-time monitoring of several process parameters and quality attributes including total cell density (TCD) and viable cell density (VCD) and concentration of glucose, lactate, ammonium, glutamine, amino acids and proteins. The resulting Raman spectra are a collection of molecular fingerprints analyzed with the use of multivariate analysis tools such as chemometric modeling. Chemometrics is used to build multivariate models of spectral variation and sample characteristics with off-line measurement in a calibration set.

Interested in monitoring your upstream cell cultures in real time? Discover how ProCellics™ Raman Analyzer with Bio4C™ PAT Raman Software, new Process Analytical Technology can help. 

  • Author
  • Recent Posts
Delphine Simiand
Latest posts by Delphine Simiand (see all)
  • Raman analytical monitoring of bioprocesses – a Process Analytical Technology (PAT) solution - September 20, 2021
+2
Share this entry
  • Share on Facebook
  • Share on Twitter
  • Share on LinkedIn
  • Share by Mail
http://emergingbiotalk.com/wp-content/uploads/2021/10/procellics-raman-anaylzer-identifier.png 650 1200 Delphine Simiand http://emergingbiotalk.com/wp-content/uploads/2020/05/emergingbiotalk-header.png Delphine Simiand2021-09-20 21:45:422021-10-22 10:28:58Raman analytical monitoring of bioprocesses – a Process Analytical Technology (PAT) solution

Biotech Resource Hub >>

  • SUBSCRIBE TO OUR BLOG
  • SUBMIT YOUR BLOG POST

Latest News

  • 5 Minutes could win you $275,000 in products and services!January 30, 2023 - 8:50 am
  • Upcoming Seminar in India: Fast track through regulatory challengesFebruary 6, 2023 - 1:10 pm
  • Enter for a chance to win $275,000 in products and services!January 9, 2023 - 11:14 am

Categories

  • Bioprocessing
  • Biosafety Testing
  • End-to-End Solutions
  • Frontpage Article
  • Gene Therapy
  • Investigator's Brochure
  • News
  • Uncategorized
  • Vaccines
  • Viral Vectors
  • Webinar

Archives

  • February 2023
  • January 2023
  • December 2022
  • November 2022
  • October 2022
  • September 2022
  • August 2022
  • July 2022
  • May 2022
  • April 2022
  • March 2022
  • February 2022
  • January 2022
  • November 2021
  • October 2021
  • September 2021
  • August 2021
  • July 2021
  • June 2021
  • May 2021
  • April 2021
  • March 2021
  • February 2021
  • January 2021
  • December 2020
  • November 2020
  • October 2020
  • September 2020
  • August 2020
  • July 2020
  • May 2020
  • April 2020
  • SUBSCRIBE TO OUR BLOG
  • SUBMIT YOUR BLOG POST

Latest News

  • 5 Minutes could win you $275,000 in products and services!January 30, 2023 - 8:50 am
  • Upcoming Seminar in India: Fast track through regulatory challengesFebruary 6, 2023 - 1:10 pm
  • Enter for a chance to win $275,000 in products and services!January 9, 2023 - 11:14 am

Categories

  • Bioprocessing
  • Biosafety Testing
  • End-to-End Solutions
  • Frontpage Article
  • Gene Therapy
  • Investigator's Brochure
  • News
  • Uncategorized
  • Vaccines
  • Viral Vectors
  • Webinar

Archives

  • February 2023
  • January 2023
  • December 2022
  • November 2022
  • October 2022
  • September 2022
  • August 2022
  • July 2022
  • May 2022
  • April 2022
  • March 2022
  • February 2022
  • January 2022
  • November 2021
  • October 2021
  • September 2021
  • August 2021
  • July 2021
  • June 2021
  • May 2021
  • April 2021
  • March 2021
  • February 2021
  • January 2021
  • December 2020
  • November 2020
  • October 2020
  • September 2020
  • August 2020
  • July 2020
  • May 2020
  • April 2020

Tags

applications bioprocessing biotech blazar Congratulations Content education Europe FDA Format gene therapy grant grants Investigator's Brochure molecule new NGS Pharmaceuticals program services symposia tags things Tone VAR2 viral assurance viral clearance virus virus detection winner

Business Hours

Our support Hotline is available 24 Hours a day: (555) 343 456 7891

  • Monday-Friday: 9am to 5pm
  • Saturday: 10am to 2pm
  • Sunday: Closed

Latest News

  • 5 Minutes could win you $275,000 in products and services!January 30, 2023 - 8:50 am
  • Upcoming Seminar in India: Fast track through regulatory challengesFebruary 6, 2023 - 1:10 pm
  • Enter for a chance to win $275,000 in products and services!January 9, 2023 - 11:14 am
  • The Future of BiopharmaDecember 6, 2022 - 4:39 am
  • Last chance: 3rd Annual Startup Symposium – Day 3November 10, 2022 - 5:39 am
  • The Lack of Funding for Women Entrepreneurs Doesn’t Stop Them from Launching – But We Still Have a Long Way to Go – And We Can Help Each Other. Assurea is ROOTING FOR YOU!October 21, 2022 - 8:27 am

Categories

  • Bioprocessing
  • Biosafety Testing
  • End-to-End Solutions
  • Frontpage Article
  • Gene Therapy
  • Investigator's Brochure
  • News
  • Uncategorized
  • Vaccines
  • Viral Vectors
  • Webinar
  • Author
  • Recent Posts
Delphine Simiand
Latest posts by Delphine Simiand (see all)
  • Raman analytical monitoring of bioprocesses – a Process Analytical Technology (PAT) solution - September 20, 2021
+2
© 2020 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. | Privacy Policy | Cookie Settings
Webinar: Developing A Scalable Upstream Bioreactor Process for Lentiviral Vector... The Making of Gene Therapy: Pioneering the Path to Cures
Scroll to top

This site uses cookies. By continuing to browse the site, you are agreeing to our use of cookies. We use cookies in our website to give you the most relevant experience. By clicking or navigating the site, you are accepting the use of all our cookies according to our Cookie Policy / Privacy Statement. You are free to decide in the Cookie Settings which categories you would like to allow. Please note that depending on what you select, the full functionality of the website may no longer be available. You may review and change your choices at any time. Further information can be found in our Privacy Policy

Accept All CookiesCookie Settings

Cookie and Privacy Settings



Cookie Preferences

When you visit our website, we store cookies on your browser to collect information. The information collected might relate to you, your preferences or your device, and is mostly used to make the site work as you expect it to and to provide a more personalized web experience. However, you can choose not to allow certain types of cookies, which may impact your experience of the site and the services we are able to offer. Click on the different category headings to find out more and change our default settings according to your preference. You cannot opt-out of our First Party Strictly Necessary Cookies as they are deployed in order to ensure the proper functioning of our website (such as prompting the cookie banner and remembering your settings, to log into your account, to redirect you when you log out, etc.). For more information about how we use and share your data, please follow this link.
Privacy Statement

Necessary

Always Active
These cookies are necessary for the website to operate. Our website cannot function without these cookies and they can only be disabled by changing your browser preferences.

Functional

These cookies enable the provision of advanced functionalities and are used for personalization. The cookies are set in particular in response to your actions and depend on your specific service requests (e.g., pop-up notification choices).Changes will take effect once you reload the page.

Targeting

These cookies may be set to learn more about your interests and show you relevant ads on other websites. These cookies work by uniquely identifying your browser and device. By integrating these cookies, we aim to learn more about your interests and your surfing behavior and to be able to place our advertising in a targeted manner.

Confirm My ChoicesAllow AllDisable All

loading

Please wait while you are redirected to the right page...

Please share your location to continue.

Check our help guide for more info.

share your location