Breaking perceptions of TFF in gene therapy manufacturing

by Ratish Krishnan | March 27, 2023


The tangential flow filtration (TFF) unit operation in viral vector manufacturing is a critical step on the path to commercialization. In this episode, Ratish Krishnan and Akshat Gupta discuss best practices – and common misconceptions – when establishing process conditions and utilizing different TFF device formats.

Below are some of the questions discussed during the podcast:

  • How and why is tangential flow filtration (TFF) used in viral vector manufacturing?
  • What are the main types of TFF formats and membranes used in viral vector purification?
  • What are the key factors and best practices when it comes to designing a TFF step?

Learn how best practices can be applied on different TFF formats to overcome common concerns in viral vectors.

Click HERE to access the full content

Senior Strategy Consultant

Ratish Krishnan is a Senior Strategy Consultant in the Novel Modalities BioProcessing group for the Americas. He is passionate about providing solutions to bring treatments to market. A Process Development Scientist by background, he has over 13 years of experience in vaccine, monoclonal antibodies and viral vector modalities from pre-clinical to late stage process characterization, validation and commercialization activities such as BLA authoring.

Related Posts

Curious to learn more on PAT, Raman Spectroscopy, and Chemometrics?

Are you curious about Process Analytical Technologies (PAT), Raman spectroscopy, chemometrics and Raman applications in bioprocessing? Watch this...

What to consider for a modular facility with closed processing

The biopharmaceutical industry trends towards new product classes and smaller batch sizes drives even a greater need for…

Watch Now: Cell & Gene Therapy On-Demand Event

Click HERE to watch the On Demand Event Abstracts Riding the Gene Therapy Roller Coaster Ratish Krishnan, Senior Strategy…

Innovative Trends That Drive AAV Capture and Empty-Full Separation

Ratish Krishnan and Oliver Rammo share their thoughts on the need for a more nuanced understanding of AAV…

Envisioning the Future of Analytical Development for Gene Therapies

As therapeutic approaches evolve, the testing and characterization methods for viral vector manufacturing need innovation to support these…

Raman analytical monitoring of bioprocesses – a Process Analytical Technology (PAT) solution

Cell culture processes are complex and highly variable in nature and yet only a handful of key parameters…

Scroll to Top