Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture

by Carole Inglevert | November 20, 2023

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Increasing plasmid demand for both production of viral and gene therapies as well as nucleic acid-based vaccines has highlighted bottlenecks in production. One bottleneck is traditional bead-based chromatography as a capture step.

To meet the needs of fast-growing markets, new production solutions are needed. These solutions must enable efficient capture of a diverse range of plasmid types and excellent clearance of bacterial host impurities, such as endotoxin.

Using a comprehensive screening approach, this case study establishes an efficient, intensified plasmid capture using convective anion exchange (AEX) chromatography and ecofriendly detergents, and provides valuable insights to intensified plasmid production.

Want to learn more? Read the Wiley article online here!

MERCK KGaA, Darmstadt, Germany
MILLIPORESIGMA
AUTHOR CONTRIBUTIONS:

André Kiesewetter: Applications Engineer, R&D Chromatography, BioProcessing

Akshat Gupta: Associate Director, Global BioPharma Center of Excellence

Anja Heinen-Kreuzig: Laboratory Technician, BioProcessing

Trish Greenhalgh: Manager, Virology, BioProcessing

Andreas Stein: Applications Engineer, Global BioPharma Center of Excellence

 

ACKNOWLEDGMENTS:

We would like to thank our

Merck KGaA, Darmstadt, Germany
MilliporeSigma
colleague Thomas Elich for valuable comments on the manuscript.

Carole Inglevert
Marketing Communication Manager

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