Increasing plasmid demand for both production of viral and gene therapies as well as nucleic acid-based vaccines has highlighted bottlenecks in production. One bottleneck is traditional bead-based chromatography as a capture step.
To meet the needs of fast-growing markets, new production solutions are needed. These solutions must enable efficient capture of a diverse range of plasmid types and excellent clearance of bacterial host impurities, such as endotoxin.
Using a comprehensive screening approach, this case study establishes an efficient, intensified plasmid capture using convective anion exchange (AEX) chromatography and ecofriendly detergents, and provides valuable insights to intensified plasmid production.
Want to learn more? Read the Wiley article online here!
André Kiesewetter: Applications Engineer, R&D Chromatography, BioProcessing
Akshat Gupta: Associate Director, Global BioPharma Center of Excellence
Anja Heinen-Kreuzig: Laboratory Technician, BioProcessing
Trish Greenhalgh: Manager, Virology, BioProcessing
Andreas Stein: Applications Engineer, Global BioPharma Center of Excellence
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